Medicaly-assisted procreation or in Vitro Fertilization (IVF) represents a method of assisted reproductive technology.
ICSI - intracytoplasmic sperm injection, is the technique of fertilization of the egg cell from the group of micro-manipulation techniques, where a single spermatozoid is injected directly into the cytoplasm of the egg cell, and thus achieves its fertilization and an embryo creation.
This method was developed in 1991 and has brought a revolution in the treatment of severe forms of male infertility, which until then had very little chance of success by applying the classical IVF procedure.
It is sufficient to have available a very low quantity of sperm using ICSI procedure.
Certain IVF clinics switched to ICSI as a standard procedure in all situations, due to work standardization and less chance of fertilization failure. However, even this may not be the best solution because the sperm selection is done mainly on the basis of morphological assessment by embryologists. This procedure does not apply to natural selection of the best spermatozoid. A number of techniques are being developed to improve the selection of the best spermatozoid (PICSI, MACS).
ICSI is used by default in following situations:
• more severe forms of pathological spermogram (reduced number, motility and proportion of normal spermatozoids)
• After all the techniques of surgical spermatozoid extraction (TESA, TESE)
• For normal spermogram when in previous cycles has been a low rate of fertilization of egg cells or even the complete absence of fertilization
• In case of large number of egg cells available, even in cases of normal spermogram, it is advisable to use fertilization of one part by IVF method and the other part using the
ICSI method, to avoid the chance of fertilization failure
With regard to the above indications, major studies of IVF or ICSI topic, showed that the success rate does not differ in one or another method, while follow-up studies of children conceived in this way have shown that the outcomes of pregnancy and childbirth are almost identical.
Nevertheless, certain studies have come to a conclusion that there is a slightly larger number of some rare anomalies following conception by ICSI procedure. It is considered that this is rather not the result of the procedure, but the situation in which a man has seriously damaged spermogram, that is a risk factor due to frequent genetic disorders behind this state.
The classic IVF represents embryonic fertilization technique - fertilization of the egg cell, in which the embryologist, in an appropriate container, and the presence of a suitable medium, leaves the egg with its surrounding and concentrate of semen, which then spontaneously fertilizes the egg cell. This technique is commonly used in situations where there is no significant disruption of semen, and its advantages are allowing "natural selection" - it is left to the most potent spermatozoid from the group to fertilize egg cell in these special conditions.Learn more
Medically-assisted procreation or In Vitro Fertilization (IVF), represents a method of assisted reproductive technology (ART). A key feature of this technology is taking both male and female gametes (semen and egg cells) in appropriate manners...
In some individuals, spermatozoa may not be present in the ejaculate. This condition is called Azoospermia. This can be either due to problems in sperm production itself or due to obstruction to the flow of semen during ejaculation. Congenital anomalies may be relatively uncommon in the general population, but can occur in up to 2 percent of infertile men. The newest of the aspiration techniques is testicular sperm retrieval (TESA – TESE).